Princess Margaret Genomics Centre

Comprehensive Genomics Platform

Get Started

Princess Margaret Genomics Centre

Comprehensive Genomics Platform

Get Started

Welcome to the Princess Margaret Research Centre

Established in 1998, the Princess Margaret Genomics Centre (PMGC) is dedicated to providing researchers (Ontario, Canada, and worldwide) access to a full spectrum of genomics services. The PMGC has solutions for a broad range of experimental needs: from wet-lab work to bioinformatics.

The PMGC is the only Canadian (and one of two in North America) 10x Genomics Certified Service Provider.
10xgenomics web page
Illumina web page

Services

DNA and RNA Sample Quantification/QC

We provide several services for characterizing your RNA and DNA samples, whether they are purified raw samples or finished libraries.

Sample Quantification and QC
  • BioAnalyzer
  • TapeStation
  • NanoDrop
  • Qubit

Illumina Sequencing and DNA Microarrays

The PMGC houses a suite of Illumina sequencing instruments including MiSeq, NextSeq 500 (x2), HiSeq 2500, and NovaSeq 6000. We perform library prep and sequencing services for a wide variety of assays. As for DNA microarrays, our service offerings are now limited to DNA microarrays provided by Illumina.

▸ Sequencing

Samples that PMGC will accept/process:

Assay Cells/Tissue RNA/DNA Samples Sequence-ready Libraries
Bulk RNA-Seq
Micro RNA-Seq
Exome-Seq
Targeted Panel
Shallow Whole Genome
shRNA/sgRNA screens
MeDIP-Seq
RRBS-Seq
ChIP-Seq
Bulk ATAC-Seq
CUT&RUN
Capture Methyl-Seq
Hi-C
Single Cell ATAC
Hi-ChIP (PLAC-Seq)
Misc Inquire
▸ DNA Microarrays
  • Infinium Methylation EPIC
  • Infinium Omni 2.5 Exome
  • Infinium GSA

Single Cell Genomics

Analyze gene expression and DNA mutations at single-cell resolution with our wide range of innovative technology offerings.

10X Genomics
  • 3' single cell gene expression
  • 5' single cell gene expression with or without Immune cell profiling (TCR/BCR)
  • Single cell ATAC-Seq
  • Feature Barcoding (single cell gene expression coupled with CRISPR)
  • Cite-Seq, Cell Haashing, and Multi-Seq is available
Fluidigm C1
  • Full length transcript sequencing
  • Biomark HD - real-time PCR for up to 96 cells x 96 transcript target
BD Rhapsody
  • 3' single cell gene expression via targeted panels
Plate-Based RNA and DNA (coming soon)
  • Smart-Seq based full length transcript sequencing
Mission Bio Tapestri (coming soon)
  • Panel-based DNA mutations and CNV
  • Cell surface protein quantification
  • Gene expression (in 2020)
Celsee Genesis (coming soon)
  • Single cell gene expression

Spatial-Omics

Description for Spatial-Omics to be completed by Troy

10X Genomics
  • Spatial Transcriptomics (Visium) is coming soon!
NanoString
  • GeoMX DSP - GeoMx digital spatial profiler

High-Throughput Nucleic Acid and Protein Quantitation

High-throughput quantitative gene and protein expression technologies.

Luminex
  • Multiplexed ELISA
  • Secreted protein quantitation
  • Protein phosphorylation quantitation
NanoString
  • Gene expression analysis
  • Single-cell gene expression analysis
  • Copy number variation analysis
  • miRNA expression analysis

Epigenomics

The PMGC has a team dedicated to testing and performing standard and emerging epigenomic and genome conformation assays. If you have an interest in an epigenomic assay not listed below, contact our team.

Available assays:
  • ChIP-Seq
  • Bulk ATAC-Seq
  • CUT&RUN
  • Capture Methyl-Seq
  • Hi-C
  • Single Cell ATAC-Seq (via 10X Genomics)
  • Hi-ChIP (PLAC-Seq)

Functional Genomics

Use genome and transcriptome editing tools such as CRISPR, CRISPRi, CRISPRa, and others to interrogate gene function.

PMGC provides:
  • Deep expertise to assist in the design and execution of successful screens and the assays for hit confirmation and validation.
  • A comprehensive suite of genome-wide and focused sgRNA libraries for mouse and human.
  • Design and construction of custom sgRNA libraries.
  • Sequencing readout of screens

FAQ

No, the PMGC performs research services only.

The PMGC is interested in participating in collaborative research projects with academic and commercial groups. We are particularly interested in collaborations which can be funded by small or large collaborative grants. A key area of focus for us is in the area of protocol and technology development. If you are interested in a collaborative project with us, please contact Troy Ketela (click name to open email).

Our shipping address is:
Princess Margaret Genomics Centre
9-601, PMCRT
101 College St.
Toronto, ON, M5G 1L7

Samples should be shipped on dry ice. We highly recommend shipping samples on a Monday or Tuesday to provide enough time for the samples to arrive before the weekend and helping to assure the dry ice will not sublime before arrival. Please note that special paperwork is required for shipping RNA or DNA from locations outside of Canada. For the Customs documentation that you will need, please download this form. Once you send us this information, the UHN Customs Officer will generate the appropriate Customs document and send it to you via e-mail. This document must be included with the waybill on your package.

Sample Submission Guidelines

Under Construction

Staff

Dr. Trevor Pugh

Scientific Director
trevor.pugh@utoronto.ca

Dr. Troy Ketela

Head of Operations
troy.ketela@uhnresearch.ca

Susan Alexander

Administrative Assistant
alexande@uhnresearch.ca

Julissa Tsao

Core Lab Manager and
Service Coordinator
(Next Generation Sequencing)
jtsao@uhnresearch.ca

Dr. Javier Diaz-Mejia

Single cell genomics bioinformatics R&D
Javier.Diaz-Mejia@uhnresearch.ca

Gurbaksh Basi

Service Coordinator
(Luminex, Fluidigm, 10X Genomics)
gbasi@uhnresearch.ca

Monika Sharma

Service Coordinator
(Agilent, Nanostring, ddPCR)
msharma@uhnresearch.ca

Mandy Xu

Service Coordinator
(Nuc-seq via 10X Genomics)
Mandy.Xu@uhnresearch.ca

Amber Azam

Research Technician
Amber.Azam@uhnresearch.ca

Iulia Cirlan

Research Technician
Iulia.Cirlan@uhnresearch.ca

Contact Us

Princess Margaret Genomics Centre

The Princess Margaret Genomics Centre is located at:

Toronto Medical Discovery Tower
101 College Street, Rm 9-301
Toronto, Ontario, M5G 1L7
Canada

Phone: (416) 581-7623
Fax: (416) 581-7430
Email: geneservice@pmgenomics.ca

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