The PMGC is an Authorized Service Provider of the Affymetrix GeneChip® platform. Affymetrix offers the flexibility to study expression, at the gene, exon, or whole-genome level, on a single system.
High quality total RNA is required for microarray experiments. We require a 260/230 ratio of greater than 1.8 and a Bioanalyzer RIN of >7.5 in order to proceed with the gene expression array experiment.
Please note that if samples do not meet the required quality standards the project will be stopped and a fee of $10/sample (to cover the sample quality assessment) will be charged. The PMGC also offers several options for dealing with degraded RNA, however, this may require a change of platform and a new quotation to be issued. We will work with you to choose the best approach if you are unable to obtain RNA of sufficient quality.
For standard Affymetrix array processing, we require a minimum of 250 ng of total RNA at >35 ng/µl. This allows for processing of the sample as well as quality control tests. If you do not have sufficient RNA, please contact us to discuss other amplification strategies such as NuGen.