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Affymetrix: Gene Expression
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The PMGC is an Authorized Service Provider of the Affymetrix GeneChip® platform. Affymetrix offers the flexibility to study expression, at the gene, exon, or whole-genome level, on a single system.

                             


Pricing: Please inquire
Pricing is highly dependent on experimental design - please allow us to help you find the most cost effective solution, take advantage of our complimentary experimental design consultation!

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Service Includes:

  • Total RNA quality checked with Agilent BioAnalyzer
  • cRNA generation
  • Hybridization, washing and scanning of arrays
  • Generation of .dat, .cel, .chp and .rpt files
  • .dat, .cel and .chp files sent to customer via the Client Portal
We also offer an optional data analysis service.


Sample Requirements:

High quality total RNA is required for microarray experiments. We require a 260/230 ratio of greater than 1.8 and a Bioanalyzer RIN of >7.5 in order to proceed with the gene expression array experiment.

Please note that if samples do not meet the required quality standards the project will be stopped and a fee of $10/sample (to cover the sample quality assessment) will be charged. The PMGC also offers several options for dealing with degraded RNA, however, this may require a change of platform and a new quotation to be issued. We will work with you to choose the best approach if you are unable to obtain RNA of sufficient quality.

For standard Affymetrix array processing, we require a minimum of 250 ng of total RNA at >35 ng/┬Ál. This allows for processing of the sample as well as quality control tests. If you do not have sufficient RNA, please contact us to discuss other amplification strategies such as NuGen.


Gene expression profiling is also available on the Illumina sequencing, Illumina array, and Agilent array platforms. Not sure which platform is best suited to your experimental needs? Click here.