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Agilent: Gene Expression
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Agilent's Dual-Mode Gene Expression Analysis Platform provides users with the flexibility to choose between one and two colour design.

The Agilent SurePrint G3 8x60K Microarrays provide extended coverage with updated transcriptome databases for mRNA targets. The human and mouse 8x60K arrays also include probes for lincRNAs (long intergenic non-coding RNAs). This new array format offers higher throughput for whole-genome profiling, improved cost savings, and lower RNA input requirements.

                                       


Pricing: Please inquire
Pricing is highly dependent on experimental design - please allow us to help you find the most cost effective solution, take advantage of our complimentary experimental design consultation!

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Service Includes:

  • Total RNA quality checked with Agilent BioAnalyzer
  • Labelling of one RNA sample per array with cyanine-3 (1-colour only) OR
  • Labelling of two RNA samples per array with cyanine-3 and cyanine-5 (one control/universal reference and one experimental) (2-colour only)
  • Hybridization/washing/scanning of arrays
  • Spot quantification (Agilent Feature Extraction)
  • Feature Extraction output files sent to customer via the Client Portal
We also offer an optional data analysis service.


Sample Requirements:

High quality total RNA is required for microarray experiments. We require a 260/230 ratio of greater than 1.8 and a Bioanalyzer RIN of >7.5 in order to proceed with the gene expression array experiment.

Please note that if samples do not meet the required quality standards the project will be stopped and a fee of $10/sample (to cover the sample quality assessment) will be charged. The PMGC also offers several options for dealing with degraded RNA, however, this may require a change of platform and a new quotation to be issued. We will work with you to choose the best approach if you are unable to obtain RNA of sufficient quality.

For standard Agilent array processing, we require a minimum of 100 ng of total RNA at >100 ng/┬Ál. This allows for processing of the sample as well as quality control tests. If you do not have sufficient RNA, please contact us to discuss other amplification strategies.


Gene expression profiling is also available on the Illumina sequencing, Illumina array, and Affymetrix array platforms. Not sure which platform is best suited to your experimental needs? Click here.