The PMGC offers a miRNA expression service using the Agilent Platform. Agilent offers human, mouse, and rat miRNA arrays. Probes are representative of mature miRNA species and allow for increased sensitivity due to their hairpin design. All Agilent miRNA array formats use an "8-up" design, where 8 arrays are present on each slide. Not all arrays need to be used; however, unused arrays cannot be saved for a later date, so for best pricing experiments should be designed to use multiples of 8 arrays.
Pricing: Please inquire
Pricing is highly dependent on experimental design - please allow us to help you find the most cost effective solution, take advantage of our complimentary experimental design consultation!
High quality total RNA is required for microarray experiments.
We require a 260/230 ratio of greater than 1.8 and a Bioanalyzer RIN of >7.5 in order to proceed with the gene expression array experiment.
Please note that if samples do not meet the required quality standards the project will be stopped and a fee of $10/sample (to cover the sample quality assessment) will be charged. The PMGC also offers several options for dealing with degraded RNA, however, this may require a change of platform and a new quotation to be issued. We will work with you to choose the best approach if you are unable to obtain RNA of sufficient quality.
For standard Agilent array processing, we require a minimum of 100 ng of total RNA at >100 ng/µl. This allows for processing of the sample as well as quality control tests. The Agilent miRNA labelling protocol recommends total RNA; however, you must be certain to use an extraction method that does not eliminate the small RNA components. At this time miRNA enriched RNA and fractionated miRNA are not recommended. Please inquire for more details.