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Functional Genomics
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The mandate of the Functional Genomics Core (FGC) at the Princess Margaret Genomics Centre is to help researchers readily access gene modulation techniques such as mammalian cell RNAi and CRISPR/Cas9. Scientists considering functional genomics applications such as pooled screens are strongly encouraged to contact the core and discuss their screening needs.


Pricing: Please inquire

Contact:


FGC provides:

  • Collaboration letters supporting grant applications with a functional genomics component
  • Deep expertise to assist in the design and execution of successful functional genomics screens and the assays for hit confirmation and validation
  • Reagents required for screening such as plasmid and lentiviral stocks of sgRNA libraries
  • Custom synthesis of sgRNA libraries
  • Illumina sequencing based sequencing readout of pooled screens (RNAi and CRISPR/Cas9)
  • A data pipeline that provides polished data with quality metrics

Genetic modulation technologies can be used for discovery of:

  • Cancer tumor-type specific essential genes
  • Genes that modulate sensitivity to molecules or environmental stresses
  • Genes that modulate metastatic potential of tumour cells
  • Genomic elements that modulate gene expression

PM researchers who develop and/or implement new functional genomics-related technologies and want to make their applications widely available through a centralized platform are also encouraged to contact the core.


Protocols:
Coming soon!


Downloads:
Pooled sgRNA screen cost calculator


Links:


Quick Questions:

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Does this platform supply shRNA pooled libraries or individual clones?

Although we can perform readout of pooled screens done with TRC shRNAs either by sequencing or GMAP microarrays, we do not distribute these shRNA reagents. We would suggest contacting the Moffat lab at the University of Toronto.

Does this platform supply individual sgRNA clones for use in CRISPR/Cas9 assays?

We do not possess an inventory of individual sgRNA clones for distribution.

What pooled sgRNA libraries do you have?

Currently, we have several genome-scale pooled sgRNA libraries for use in CRISPR/Cas9 screens. We’re also in the process of building a set of smaller functionally focused pooled libraries. Contact us to see what reagents we have and which might best suit your purpose.

I’d like to make a custom content sgRNA pool but don’t know how.

Contact the core and we can discuss protocols and the possibility of custom contract synthesis of sgRNA pools.

I’d like my lab to perform some CRISPR/Cas9 screens but have no experience in the art. Can I get advice?

Absolutely! The platform provides free consultation to PM researchers (fees may apply for PIs external to PM). This service includes screen design, analysis and interpretation.

I need help in designing screens as part of a grant application and/or need a letter of collaboration.

No problem, contact the platform. We’re happy to help.