The mandate of the Functional Genomics Core (FGC) at the Princess Margaret Genomics Centre is to help researchers readily access gene modulation techniques such as mammalian cell RNAi and CRISPR/Cas9. Scientists considering functional genomics applications such as pooled screens are strongly encouraged to contact the core and discuss their screening needs.
Pricing: Please inquire
Genetic modulation technologies can be used for discovery of:
PM researchers who develop and/or implement new functional genomics-related technologies and want to make their applications widely available through a centralized platform are also encouraged to contact the core.
Pooled sgRNA screen cost calculator
Although we can perform readout of pooled screens done with TRC shRNAs either by sequencing or GMAP microarrays, we do not distribute these shRNA reagents. We would suggest contacting the Moffat lab at the University of Toronto.
We do not possess an inventory of individual sgRNA clones for distribution.
Currently, we have several genome-scale pooled sgRNA libraries for use in CRISPR/Cas9 screens. We’re also in the process of building a set of smaller functionally focused pooled libraries. Contact us to see what reagents we have and which might best suit your purpose.
Contact the core and we can discuss protocols and the possibility of custom contract synthesis of sgRNA pools.
Absolutely! The platform provides free consultation to PM researchers (fees may apply for PIs external to PM). This service includes screen design, analysis and interpretation.
No problem, contact the platform. We’re happy to help.