The PMGC provides sample quantification service on either the NanoDrop ND-1000, the Qbit® 2.0 Fluorometer (Invitrogen), or the Infinite® 200 PRO NanoQuant (Tecan).
The NanoDrop ND-1000 Spectrophotometer is capable of full spectrum UV-Vis absorbance analyzes (220-750 nm) using 1 µl of sample. Its patented sample retention technology uses fiber optic connectors to hold the sample in place during measurement. For dsDNA, the concentration range of the sample should be 2-3700 ng/µl for accurate quantification.
The Qbit® instrument from Invitrogen quantitates DNA, RNA, and protein with unprecedented accuracy, sensitivity, and simplicity. It is designed for precious samples that are rare or difficult to process, and applications requiring precise measurement such as real-time PCR and deep sequencing. Quantifies RNA samples in the range of 250 pg/µl to 100 ng/µl and dsDNA in the range of 100 pg/µl to 1000 ng/µl.
The Infinite® 200 PRO NanoQuant is the first multimode microplate reader to be developed specifically for absorbance applications with low sample volumes. It delivers excellent sensitivity, multiplexing capability and high format flexibility including microplates, cuvettes and the NanoQuant Plate for low concentration and small sample size. This instrument is used for more high-throughput sample quantification needs (more than 32 samples at a time).
Cost: $5 per sample for NanoDrop or Qbit quantification.
Please inquire regarding Tecan Infinite® 200 PRO NanoQuant quantification.
Turnaround time: 2 business days or less
For nucleic acids, the minimum volume required is 1 µl and the concentration should be between 2-3700 ng/µl (NanoDrop) and either 0.1-1000 ng/µl for dsDNA or 0.25-100 ng/µl for RNA (Qbit).
For protein samples, 2 µl of sample is required.
Customers are asked to bring/submit at least 2 µl for nucleic acid quantitation (NanoDrop or Qbit) and 4 µl for protein quantitation (NanoDrop only).
No, the sample cannot be retrieved following its measurement.
The NanoDrop ND-1000 spectrophotometer is able to perform full spectrum UV-Vis absorbance analyses (220-750 nm). Using the microarray application, the concentration of nucleic acid and up to 2 dyes can be calculated and displayed at the same time.
There are a few solvent restrictions when using the NanoDrop. For example, hydrofluoric acid should definitely not be used. Samples dissolved in high vapour pressure solvents such as acetone can be measured but only using the short path length to reduce evaporation. Please contact us prior to using the NanoDrop if you are using non-standard buffers/solvents.