Established in 1998, the Princess Margaret Genomics Centre (PMGC) is dedicated to providing access to cost-effective and high quality genomics services. We service local, national and international clients from both academic and commercial research organizations. The PMGC has solutions for a broad range of experimental needs. Our specialities include standard and custom-tailored DNA sequencing techniques using Illumina technologies, epigenomics, panel-based profiling (Luminex and Nanostring) and functional genomics. Single cell genomics applications on a variety of popular platforms is one of our most popular services. We also specialize in unique and custom techniques that aren’t found at most service providers. If you are looking for a service that you don’t see on our website, inquire!
We provide several services for characterizing your RNA and DNA
samples, whether they are purified nucleic acids, sequence ready
libraries, or anything in between.
To submit samples for BioAnalyzer, please download
this form
and submit with your samples. For other quantification services
please contact a PMGC team member.
The PMGC houses a full suite of Illumina sequencing instruments including MiSeq (x2), NextSeq 500, NextSeq 2000, NovaSeq 6000 and NovaSeq X Plus. We perform library prep and sequencing services for a wide variety of assays. As for DNA microarrays, our service offerings are currently limited to DNA microarrays provided by Illumina.
Sequence-ready library types accepted:
| Whole Exome | ChIP | Micro-RNA |
| Whole Genome | ATAC | Cut&Run |
| Target capture panels | Methyl-Capture | Low/Hi-C |
| Bulk RNA Seq | MeDIP | 10x Genomics (all types) |
| RRBS | WGBS | CRISPR-Cas9 screens |
| Custom primer configurations | RIP-Seq | Inquire for others |
Library types that we can prepare and sequence from your nucleic acid samples:
| Whole Exome | Whole Genome | Target capture panels |
| CRISPR-Cas9 screens | Bulk RNA-Seq | ChIP (pulldown by client) |
| Micro-RNA | Inquire for others |
Library types that we can prepare and sequence from cells/tissue:
| ATAC-Seq | Hi-C | CUT&RUN |
| Hi-Chip | Capture Methyl-Seq | Inquire for others |
Analyze gene expression and DNA mutations at single-cell resolution with our wide range of innovative technology offerings.
Spatial Omics integrates microscopy of tissue sections with readout of the transcriptome, proteins, and more. This allows one to collect information about the positional and phenotypic aspects of the cells whose transcriptional patterns are being interrogated. This novel technology is an extremely powerful extension of conventional histology!
Quantitative panel-based gene and protein expression assays from Nanostring and Luminex.
The PMGC has a team dedicated to validating and performing standard and emerging epigenomic and genome conformation assays. If you have an interest in an assay not listed below, contact our team to discuss.
Use genome and transcriptome editing tools such as CRISPR, CRISPRi, CRISPRa, and others to interrogate gene function.
No, the PMGC performs research services only.
The PMGC is interested in participating in collaborative research projects with academic and commercial groups. We are particularly interested in collaborations which can be funded by small or large collaborative grants. A key area of focus for us is in the area of protocol and technology development. If you are interested in a collaborative project with us, please contact Troy Ketela (click name to open email).
Shipping samples:
Please ship samples out on a
Monday/Tuesday to prevent weekend
delays and ensure arrival during working days. For
frozen samples, transport in an appropriate container
with sufficient dry ice to ensure dry ice will remain
for the duration of the delivery. For international
clients, we recommend shipping with
World Courier
for tissues/cells. Within Canada, or if shipping
DNA/RNA, we recommend FedEX Next Day Priority services.
Shipping address:
Customs Information
Please note that special paperwork is required for
shipping samples, including RNA or DNA, from locations
outside of Canada. For the Customs documentation, please
liaise with your PMGC contact and shipping vendor for
the information required. The UHN Customs Officer will
generate the appropriate Customs document and send it to
you via e-mail.
The Customs document must be included with the
waybill on your package.
Please reach out via email either directly to a PMGC team member, or to geneservice@pmgenomics.ca.
The Princess Margaret Genomics Centre is located at:
Princess Margaret Cancer Research Tower
101 College Street, Rm 9-805
Toronto, Ontario, M5G 1L7
Canada
Phone:
(416) 634-8816
Email:
geneservice@pmgenomics.ca
LinkedIn:
princess-margaret-genomics-centre
The Brain Single Cell Initiative is making state-of-the-art
single-cell and spatial genomics technology available to brain
researchers through the Princess Margaret Genomics Centre.
Click
here to learn more.
