Princess Margaret Genomics Centre

Comprehensive Genomics Solutions

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Welcome to the Princess Margaret Genomics Centre

Established in 1998, the Princess Margaret Genomics Centre (PMGC) is dedicated to providing access to cost-effective and high quality genomics services. We service local, national and international clients from both academic and commercial research organizations. The PMGC has solutions for a broad range of experimental needs. Our specialities include standard and custom-tailored DNA sequencing techniques using Illumina technologies, epigenomics, panel-based profiling (Luminex and Nanostring) and functional genomics. Single cell genomics applications on a variety of popular platforms is one of our most popular services. We also specialize in unique and custom techniques that aren’t found at most service providers. If you are looking for a service that you don’t see on our website, inquire!

The PMGC is the only Canadian (and one of two in North America) 10x Genomics-Certified Service Provider.
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Services

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DNA and RNA Sample Quantification/QC

We provide several services for characterizing your RNA and DNA samples, whether they are purified nucleic acids, sequence ready libraries, or anything in between.
To submit samples for this service please register for an account in our new customer management system (Stratocore PPMS) here and use the "order" function to create an order to reflect your requirements.

Sample Quantification and QC
  • BioAnalyzer
  • TapeStation
  • NanoDrop
  • Qubit
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Illumina Sequencing and DNA Microarrays

The PMGC houses a full suite of Illumina sequencing instruments including MiSeq, NextSeq 500 (x2), HiSeq 2500, and NovaSeq 6000. We perform library prep and sequencing services for a wide variety of assays. As for DNA microarrays, our service offerings are currently limited to DNA microarrays provided by Illumina.

▸ Sequencing

Sequence-ready library types accepted:

Whole Exome ChIP Micro-RNA
Whole Genome ATAC Cut&Run
Target capture panels Methyl-Capture Low/Hi-C
Bulk RNA Seq MeDIP 10X Genomics (all types)
RRBS WGBS CRISPR-Cas9 screens
Custom primer configurations RIP-Seq Inquire for others

Library types that we can prepare and sequence from your nucleic acid samples:

Whole Exome Whole Genome Target capture panels
CRISPR-Cas9 screens Bulk RNA-Seq ChIP (pulldown by client)
Micro-RNA Inquire for others

Library types that we can prepare and sequence from cells/tissue:

ATAC-Seq Hi-C CUT&RUN
Hi-Chip Capture Methyl-Seq Inquire for others
▸ DNA Microarrays
  • Infinium Methylation EPIC
  • Infinium Omni 2.5 Exome
  • Infinium GSA
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Single Cell Genomics

Analyze gene expression and DNA mutations at single-cell resolution with our wide range of innovative technology offerings.

10X Genomics
  • 3' single cell gene expression
  • 5' single cell gene expression with or without Immune cell profiling (TCR/BCR)
  • Single cell ATAC-Seq
  • Single Cell Multiome (same-cell ATAC+gene expression)
  • Feature Barcoding (single cell gene expression coupled with CRISPR)
  • Single Cell Targeted Panels
  • Cite-Seq, Cell Hashing, and Multi-Seq is available
Fluidigm C1
  • Full length transcript sequencing
  • Biomark HD - real-time PCR for up to 96 cells x 96 transcript targets
BD Rhapsody
  • 3' single cell gene expression via targeted panels
Mission Bio Tapestri
  • Panel-based DNA mutations and CNV with the option to quantify cell surface proteins
  • Gene expression (coming soon)
Celsee Genesis
  • Gene expression
  • Inquire about other workflows
Plate-Based single cell mRNA and DNA sequencing (coming soon)
  • Smart-Seq based full length transcript sequencing
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Spatial-Omics

Spatial-Omics integrates microscopy of tissue sections with readout of the transcriptome. This allows one to collect information about the positional and phenotypic aspects of the cells whose transcriptional patterns are being interrogated. This novel technology is an extremely powerful extension of conventional histology!

10X Genomics
  • Visium spatial transcriptomics
NanoString
  • We offer onboarding of samples for GeoMX spatial profiling to be performed by Nanostring at their facilities
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Nanostring and Luminex

Quantitative panel-based gene and protein expression assays from Nanostring and Luminex.

Luminex
  • Multiplexed ELISA
  • Secreted protein quantitation
  • Protein phosphorylation quantitation
NanoString
  • Gene expression analysis
  • Single-cell gene expression analysis
  • Copy number variation analysis
  • miRNA expression analysis
  • PlexSets for high-throughput applications
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Epigenomics

The PMGC has a team dedicated to validating and performing standard and emerging epigenomic and genome conformation assays. If you have an interest in an assay not listed below, contact our team to discuss.

Available assays:
  • ChIP-Seq
  • Bulk ATAC-Seq
  • CUT&RUN
  • Capture Methyl-Seq
  • Hi-C
  • Single Cell ATAC-Seq (via 10X Genomics)
  • Hi-ChIP (PLAC-Seq)
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Functional Genomics

Use genome and transcriptome editing tools such as CRISPR, CRISPRi, CRISPRa, and others to interrogate gene function.

PMGC provides:
  • Deep expertise to assist/advise in the design and execution of successful screens and the assays for hit confirmation and validation.
  • A comprehensive collection of genome-wide and focused sgRNA libraries for mouse and human.
  • Design and construction of custom sgRNA libraries.
  • Sequencing readout of CRISPR-based screens

FAQ

No, the PMGC performs research services only.

The PMGC is interested in participating in collaborative research projects with academic and commercial groups. We are particularly interested in collaborations which can be funded by small or large collaborative grants. A key area of focus for us is in the area of protocol and technology development. If you are interested in a collaborative project with us, please contact Troy Ketela (click name to open email).

Our shipping address is:
Princess Margaret Genomics Centre
9-601, PMCRT
101 College St.
Toronto, ON, M5G 1L7

Samples should be shipped on dry ice. We highly recommend shipping samples on a Monday or Tuesday to provide enough time for the samples to arrive before the weekend and helping to assure the dry ice will not sublime before arrival. Please note that special paperwork is required for shipping RNA or DNA from locations outside of Canada. For the Customs documentation that you will need, please download this form. Once you send us this information, the UHN Customs Officer will generate the appropriate Customs document and send it to you via e-mail. This document must be included with the waybill on your package.

We are in the process of switching to use a new customer management system (Stratocore PPMS). Currently, this system can be used to order/submit samples for our DNA and RNA Sample Quantification/QC services by registering for an account here. We will fully transition to this software soon, until then please email geneservice@pmgenomics.ca to receive a quotation for all other services.

Sample Submission Guidelines

Under Construction

Team

Dr. Troy Ketela

UHN Staff Scientist II,
Head of Operations

Dr. Trevor Pugh

Senior Scientist,
Princess Margaret Cancer Centre

Susan Alexander

Administrative Assistant

Gurbaksh Basi

10X Genomics single cell,
Fluidigm,
Mission Bio Tapestri

Iulia Cirlan

10X Genomics single cell,
Spatial Transcriptomics

Nicholas Khuu

Epigenomics,
Next-gen sequencing

Dr. Elias Orouji

UHN Staff Scientist,
Epigenomics lead

Monika Sharma

Nanostring,
Luminex,
Micro RNA sequencing

Roxana Shen

Epigenomics

Julissa Tsao

Lab Manager,
Next-gen sequencing,
Illumina arrays

Mandy Xu

10X Genomics nuclei seq

Contact Us

Princess Margaret Genomics Centre

The Princess Margaret Genomics Centre is located at:

Toronto Medical Discovery Tower
101 College Street, Rm 9-301
Toronto, Ontario, M5G 1L7
Canada

Phone: (416) 581-7623
Fax: (416) 581-7430
Email: geneservice@pmgenomics.ca

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