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Princess Margaret Genomics Centre

Comprehensive Genomics Solutions

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Welcome to the Princess Margaret Genomics Centre

Established in 1998, the Princess Margaret Genomics Centre (PMGC) is dedicated to providing access to cost-effective and high quality genomics services. We service local, national and international clients from both academic and commercial research organizations. The PMGC has solutions for a broad range of experimental needs. Our specialities include standard and custom-tailored DNA sequencing techniques using Illumina technologies, epigenomics, panel-based profiling (Luminex and Nanostring) and functional genomics. Single cell genomics applications on a variety of popular platforms is one of our most popular services. We also specialize in unique and custom techniques that aren’t found at most service providers. If you are looking for a service that you don’t see on our website, inquire!

PMGC is a 10X Genomics-Certified service provider.

News and Updates

Read all about what we've been up to
picture of SMRT Grant Program Announcement
March 17, 2022

2022 HiFi For Accuracy SMRT Grant Program

Apply before 5:00 PDT on April 1, 2022, for your chance to win free sequencing.
Learn more and apply at

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February 16, 2022

Team PMGC marks the 500th successful flow cell run on our NovaSeq 6000 instrument


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DNA and RNA Sample Quantification/QC

We provide several services for characterizing your RNA and DNA samples, whether they are purified nucleic acids, sequence ready libraries, or anything in between.
To submit samples for BioAnalyzer, please download this form and submit with your samples. For other quantification services please contact a PMGC team member.

Sample Quantification and QC
  • BioAnalyzer
  • TapeStation
  • NanoDrop
  • Qubit
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Illumina Sequencing and DNA Microarrays

The PMGC houses a full suite of Illumina sequencing instruments including MiSeq (x2), NextSeq 500 (x2), and NovaSeq 6000. Mid-2023, we will be installing an Illumina NovaSeq X Plus. We perform library prep and sequencing services for a wide variety of assays. As for DNA microarrays, our service offerings are currently limited to DNA microarrays provided by Illumina.

▸ Sequencing

Sequence-ready library types accepted:

Whole Exome ChIP Micro-RNA
Whole Genome ATAC Cut&Run
Target capture panels Methyl-Capture Low/Hi-C
Bulk RNA Seq MeDIP 10X Genomics (all types)
Custom primer configurations RIP-Seq Inquire for others

Library types that we can prepare and sequence from your nucleic acid samples:

Whole Exome Whole Genome Target capture panels
CRISPR-Cas9 screens Bulk RNA-Seq ChIP (pulldown by client)
Micro-RNA Inquire for others

Library types that we can prepare and sequence from cells/tissue:

Hi-Chip Capture Methyl-Seq Inquire for others
▸ DNA Microarrays
  • Infinium Methylation EPIC
  • Infinium Omni 2.5 Exome
  • Infinium GSA
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Single Cell Genomics

Analyze gene expression and DNA mutations at single-cell resolution with our wide range of innovative technology offerings.

10X Genomics
  • 3' single cell gene expression
  • 5' single cell gene expression with or without immune cell V(D)J profiling (TCR/BCR)
  • Single nucleus ATAC-Seq
  • Multiome - same-nucleus ATAC+gene expression profiling
  • Single cell CRISPR 3' or 5'
  • Single Cell Targeted Panels
  • Feature Barcoding (Cite-Seq), Cell Hashing, and CellPlex (sample multiplexing) are available
  • Flex - profiling of cells and nuclei that have been formaldehyde-fixed
Parse Biosciences
  • Highly multiplexed RNA sequencing on nuclei or whole cells
Fluidigm C1
  • Full-length transcript sequencing
  • Biomark HD - real-time PCR for up to 96 cells x 96 transcript targets
Mission Bio Tapestri
  • Panel-based DNA mutations and CNV with the option to quantify cell surface proteins
Plate-based single cell mRNA sequencing
  • SeqWell plexWell Rapid Single Cell plate-based full length transcript sequencing
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Spatial Omics

Spatial Omics integrates microscopy of tissue sections with readout of the transcriptome, proteins, and more. This allows one to collect information about the positional and phenotypic aspects of the cells whose transcriptional patterns are being interrogated. This novel technology is an extremely powerful extension of conventional histology!

10X Genomics
  • Visium spatial transcriptomics (fresh-frozen and FFPE)
  • Visium CytAssist - the improved 2nd generation Visium workflow for FFPE and fresh-frozen tissues
  • Xenium - sub-cellular resolution transcriptional profiling (June 2023)
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Nanostring and Luminex

Quantitative panel-based gene and protein expression assays from Nanostring and Luminex.

  • Multiplexed ELISA
  • Secreted protein quantitation
  • Protein phosphorylation quantitation
  • Gene expression analysis
  • Single-cell gene expression analysis
  • Copy number variation analysis
  • miRNA expression analysis
  • PlexSets for high-throughput applications
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The PMGC has a team dedicated to validating and performing standard and emerging epigenomic and genome conformation assays. If you have an interest in an assay not listed below, contact our team to discuss.

Available assays:
  • ChIP-Seq
  • Bulk ATAC-Seq
  • Capture Methyl-Seq
  • Hi-C
  • Hi-ChIP (PLAC-Seq)
  • EM Seq (June 2023)
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Functional Genomics

Use genome and transcriptome editing tools such as CRISPR, CRISPRi, CRISPRa, and others to interrogate gene function.

PMGC provides:
  • Deep expertise to assist/advise in the design and execution of successful screens and the assays for hit confirmation and validation.
  • A comprehensive collection of genome-wide and focused sgRNA libraries for mouse and human.
  • Design and construction of custom sgRNA libraries.
  • Sequencing readout of CRISPR-based screens


No, the PMGC performs research services only.

The PMGC is interested in participating in collaborative research projects with academic and commercial groups. We are particularly interested in collaborations which can be funded by small or large collaborative grants. A key area of focus for us is in the area of protocol and technology development. If you are interested in a collaborative project with us, please contact Troy Ketela (click name to open email).

Our shipping address is:
Princess Margaret Genomics Centre
9-601, PMCRT
101 College St.
Toronto, ON, M5G 1L7

Samples should be shipped on dry ice. We highly recommend shipping samples on a Monday or Tuesday to provide enough time for the samples to arrive before the weekend and helping to assure the dry ice will not sublime before arrival. Please note that special paperwork is required for shipping RNA or DNA from locations outside of Canada. For the Customs documentation that you will need, please download this form . Once you send us this information, the UHN Customs Officer will generate the appropriate Customs document and send it to you via e-mail. This document must be included with the waybill on your package.

Please reach out via email either directly to a PMGC team member, or to

Sample Submission Guidelines

Under Construction


Dr. Troy Ketela

UHN Staff Scientist II,
Head of Operations - PMGC

Dr. Trevor Pugh

Senior Scientist,
Princess Margaret Cancer Centre,
Scientific Director - PMGC

Farzaneh Aboualizadeh

10X Genomics spatial 'omics,
Mission Bio Tapestri

Amina Abow

10X Genomics spatial 'omics,
Mission Bio Tapestri

Wafa Al-Ameri

10X Genomics single nuclei profiling

Gurbaksh Basi

10X Genomics single cell profiling,

Tanja Durbic

Functional Genomics (CRISPR),
Plate-based single cell genomics

Dr. Ka Man (Carman) Ip

High Throughput Screening

Jennifer Law

Research Associate/Project Manager,
Brain Single Cell Initiative

Samantha Lee

10X Genomics single cell

Tanya Mohanta

10X Genomics single cell profiling,

Genevieve Mailhot

10X Genomics Flex,
10X Genomics single nuclei profiling

Dr. Elias Orouji

UHN Staff Scientist,
Epigenomics Lead

Johanna Regala

10X Genomics single nuclei profiling

Jawad Salehi

Next-generation sequencing

Monika Sharma

Micro RNA sequencing

Julissa (Julie) Tsao

Lab Manager,
Next-generation sequencing,
Illumina arrays

Laura Vu

Administrative Assistant

Contact Us

Princess Margaret Genomics Centre

The Princess Margaret Genomics Centre is located at:

Princess Margaret Cancer Research Tower
101 College Street, Rm 9-301
Toronto, Ontario, M5G 1L7

Phone: (416) 581-7623
Fax: (416) 581-7430
LinkedIn: princess-margaret-genomics-centre

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